Description：Rabbit polyclonal antibody to FGFR1 (Phospho-Y654)Immunogen：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y654 of human FGFR1 protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：FGFR1Alternative Names：BFGFR; CEK; FGFBR; FLG; FLT2; HBGFR; Fibroblast growth factor receptor 1; FGFR-1; Basic fibroblast growth factor receptor 1; BFGFR; bFGF-R-1; Fms-like tyrosine kinase 2; FLT-2; N-sam; Proto-oncogene c-Fgr; CD331 Entrez Gene (Human)： 2260; Entrez Gene (Mouse)： 14182; Entrez Gene (Rat)： 79114; SwissProt (Human)： P11362; SwissProt (Mouse)： P16092; SwissProt (Rat)： Q04589; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of FGFR1 (Phospho-Y654) expression in mouse testis (A) whole cell lysates. (Predicted band size: 91 kD; Observed band size: 120 kD)
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  Immunofluorescent analysis of FGFR1 (Phospho-Y654) staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.