Description：Recombinant rabbit monoclonal antibody to SLC7A11Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within human SLC7A11 protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：MonoclonalForm：Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200), IP (1/10 - 1/50)Gene Symbol：SLC7A11Alternative Names：Cystine/glutamate transporter; Amino acid transport system xc-; Calcium channel blocker resistance protein CCBR1; Solute carrier family 7 member 11; xCT Entrez Gene (Human)： 23657; Entrez Gene (Mouse)： 26570; SwissProt (Human)： Q9UPY5; SwissProt (Mouse)： Q9WTR6; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of SLC7A11 expression in Jurkat (A), Hela (B), K562 (C), mouse liver (D), mouse muscle (E), rat liver (F), rat muscle (G) whole cell lysates. (Predicted band size: 55 kD; Observed band size: 55 kD)
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  Immunofluorescent analysis of SLC7A11 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).