Description：Rabbit polyclonal antibody to ARFGAP2Immunogen：Recombinant fusion protein of human ARFGAP2. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol：ARFGAP2Alternative Names：ZNF289; ADP-ribosylation factor GTPase-activating protein 2; ARF GAP 2; GTPase-activating protein ZNF289; Zinc finger protein 289 Entrez Gene (Human)： 84364; Entrez Gene (Mouse)： 77038; Entrez Gene (Rat)： 362162; SwissProt (Human)： Q8N6H7; SwissProt (Mouse)： Q99K28; SwissProt (Rat)： Q3MID3; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ARFGAP2 expression in THP1 (A), mouse kidney (B), rat lung (C) whole cell lysates. (Predicted band size: 56 kD; Observed band size: 57 kD)
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  Immunofluorescent analysis of ARFGAP2 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).