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CQA3759
  • Western blot analysis of Centaurin-alpha-1 expression in Jurkat (A), mouse small intestine (B), rat kidney (C) whole cell lysates. (Predicted band size: 35; 43; 44 kD; Observed band size: 43 kD)
  • Immunofluorescent analysis of Centaurin-alpha-1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
Product Name:Anti-Centaurin-alpha-1 Antibody
Cat No:CQA3759
Source:Rabbit
Reactivity:H, M, R
Applications:WB, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
50 μl
150
30 μl
110
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Description:Rabbit polyclonal antibody to Centaurin-alpha-1
Immunogen:Recombinant fusion protein of human Centaurin-alpha-1. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Gene Symbol:ADAP1
Alternative Names:CENTA1; Arf-GAP with dual PH domain-containing protein 1; Centaurin-alpha-1; Cnt-a1; Putative MAPK-activating protein PM25
Entrez Gene (Human): 11033;
SwissProt (Human): O75689;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of Centaurin-alpha-1 expression in Jurkat (A), mouse small intestine (B), rat kidney (C) whole cell lysates. (Predicted band size: 35; 43; 44 kD; Observed band size: 43 kD)
  • Immunofluorescent analysis of Centaurin-alpha-1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
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