Description：Rabbit polyclonal antibody to AKAP4Immunogen：Recombinant protein of human AKAP4. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol：AKAP4Alternative Names：AKAP82; A-kinase anchor protein 4; AKAP-4; A-kinase anchor protein 82 kDa; AKAP 82; hAKAP82; Major sperm fibrous sheath protein; HI; Protein kinase A-anchoring protein 4; PRKA4 Entrez Gene (Mouse)： 11643; Entrez Gene (Rat)： 79254; SwissProt (Mouse)： Q60662; SwissProt (Rat)： O35774; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of AKAP4 expression in mouse testis (A), rat testis (B) whole cell lysates. (Predicted band size: 93; 94 kD; Observed band size: 82 kD)
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  Immunofluorescent analysis of AKAP4 staining in mouse testis. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).