Description：Rabbit polyclonal antibody to F4/80Immunogen：Recombinant protein of human F4/80. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：ADGRE1Alternative Names：EMR1; TM7LN3; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-like module receptor 1; EMR1 hormone receptor; Adhesion G protein-coupled receptor E1; F4/80 Entrez Gene (Human)： 2015; Entrez Gene (Mouse)： 13733; Entrez Gene (Rat)： 316137; SwissProt (Human)： Q14246; SwissProt (Mouse)： Q61549; SwissProt (Rat)： Q5Y4N8; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of F4/80 expression in Hela (A), mouse liver (B) whole cell lysates. (Predicted band size: 78; 82; 90; 95; 97 kD; Observed band size: 160 kD)
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  Immunofluorescent analysis of F4/80 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).