Description：Rabbit polyclonal antibody to GHRH ReceptorImmunogen：KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GHRH Receptor. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Gene Symbol：GHRHRAlternative Names：Growth hormone-releasing hormone receptor; GHRH receptor; Growth hormone-releasing factor receptor; GRF receptor; GRFR Entrez Gene (Human)： 2692; Entrez Gene (Mouse)： 14602; Entrez Gene (Rat)： 25321; SwissProt (Human)： Q02643; SwissProt (Mouse)： P32082; SwissProt (Rat)： Q02644; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of GHRH Receptor expression in mouse kidney (A), rat kidney (B) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 47; 55 kD)
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  Immunofluorescent analysis of GHRH Receptor staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).