- Cat No.
CRP2360
- Source
E. coli
- Reactivity
Human
- Applications
E; WB; SDS-PAGE; MS
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Application key: E- ELISA, WB- Western blot, IH- Immunohistochemistry, IF- Immunofluorescence, FC- Flow cytometry, IC- Immunocytochemistry, IP- Immunoprecipitation, ChIP- Chromatin Immunoprecipitation, EMSA- Electrophoretic Mobility Shift Assay, BL- Blocking, SE- Sandwich ELISA, CBE- Cell-based ELISA, RNAi- RNA interference
Species reactivity key: H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish
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General information
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| Description |
Recombinant Human SUMO1 Protein is produced by our E. coli expression system and the target gene encoding Met1?Val101 is expressed with a 6His tag at the N-terminus. |
| Form |
Lyophilized from a 0.2 uM filtered solution of 50mM Tris-HCl; 100mM NaCl; 1mM DTT; pH 8.5. |
| Gene Symbol |
SUMO1 |
| Alternative Names |
SMT3C; SMT3H3; UBL1; Small ubiquitin-related modifier 1; SUMO-1; GAP-modifying protein 1; GMP1; SMT3 homolog 3; Sentrin; Ubiquitin-homology domain protein PIC1; Ubiquitin-like protein SMT3C; Smt3C; Ubiquitin-like protein UBL1 |
| Entrez Gene (Human) |
7341 |
| SwissProt (Human) |
P63165 |
| Purity |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
| Chemical Structure |
MGSSHHHHHH SSGLVPRGSH MSDQEAKPST EDLGDKKEGE YIKLKVIGQD SSEIHFKVKM TTHLKKLKES YCQRQGVPMN SLRFLFEGQR IADNNTPKEL GMEEEDVIEV YQEQTGGHST V |
| Quality Control |
Endotoxin: Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. |
| Directions for Use |
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Storage/Stability |
Lyophilized protein should be stored at -20 deg C; though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 deg C for 2-7 days. Aliquots of reconstituted samples are stable at -20 deg C for 3 months. |
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