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Recombinant Human SHMT1 Protein

  • Cat No.
    CRP2302
  • Source
    Human cells
  • Reactivity
    Human
  • Applications
    E; WB; SDS-PAGE; MS
Application key: E- ELISA, WB- Western blot, IH- Immunohistochemistry, IF- Immunofluorescence, FC- Flow cytometry, IC- Immunocytochemistry, IP- Immunoprecipitation, ChIP- Chromatin Immunoprecipitation, EMSA- Electrophoretic Mobility Shift Assay, BL- Blocking, SE- Sandwich ELISA, CBE- Cell-based ELISA, RNAi- RNA interference

Species reactivity key: H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish
General information
Specifications
Data
References
Related Products
Description Recombinant Human SHMT1 Protein is produced by our mammalian expression system and the target gene encoding Met3-Phe483 is expressed with a 6His tag at the C-terminus.
Form Lyophilized from a 0.2 uM filtered solution of 20mM PB; 150mM NaCl; pH 7.4.
Gene Symbol SHMT1
Alternative Names Serine hydroxymethyltransferase cytosolic; SHMT; Glycine hydroxymethyltransferase; Serine methylase
Entrez Gene (Human) 6470
SwissProt (Human) P34896
Purity Greater than 95% as determined by reducing SDS-PAGE.
Chemical Structure MPVNGAHKDA DLWSSHDKML AQPLKDSDVE VYNIIKKESN RQRVGLELIA SENFASRAVL EALGSCLNNK YSEGYPGQRY YGGTEFIDEL ETLCQKRALQ AYKLDPQCWG VNVQPYSGSP ANFAVYTALV EPHGRIMGLD LPDGGHLTHG FMTDKKKISA TSIFFESMPY KVNPDTGYIN YDQLEENARL FHPKLIIAGT SCYSRNLEYA RLRKIADENG AYLMADMAHI SGLVAAGVVP SPFEHCHVVT TTTHKTLRGC RAGMIFYRKG VKSVDPKTGK EILYNLESLI NSAVFPGLQG GPHNHAIAGV AVALKQAMTL EFKVYQHQVV ANCRALSEAL TELGYKIVTG GSDNHLILVD LRSKGTDGGR AEKVLEACSI ACNKNTCPGD RSALRPSGLR LGTPALTSRG LLEKDFQKVA HFIHRGIELT LQIQSDTGVR ATLKEFKERL AGDKYQAAVQ ALREEVESFA SFFPLPGLPD FVDHHHHHH
Quality Control Endotoxin: Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Directions for Use Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage/Stability Lyophilized protein should be stored at -20 deg C; though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 deg C for 2-7 days. Aliquots of reconstituted samples are stable at -20 deg C for 3 months.
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For research purposes only, not for human use
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