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Recombinant E. coli NAGK Protein

  • Cat No.
    CRP1987
  • Source
    E. coli
  • Reactivity
    E. coli
  • Applications
    E; WB; SDS-PAGE; MS
Application key: E- ELISA, WB- Western blot, IH- Immunohistochemistry, IF- Immunofluorescence, FC- Flow cytometry, IC- Immunocytochemistry, IP- Immunoprecipitation, ChIP- Chromatin Immunoprecipitation, EMSA- Electrophoretic Mobility Shift Assay, BL- Blocking, SE- Sandwich ELISA, CBE- Cell-based ELISA, RNAi- RNA interference

Species reactivity key: H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish
General information
Specifications
Data
References
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Description Recombinant E. coli NAGK Protein is produced by our E. coli expression system and the target gene encoding Met1-Asp303 is expressed with a 6His tag at the C-terminus.
Form Supplied as a 0.2 uM filtered solution of 20mM Tris-HCl; 100mM NaCl; 2mM DTT; 20% Glycerol; pH 8.0.
Gene Symbol NAGK
Alternative Names ycfX; N-acetyl-D-glucosamine kinase; GlcNAc kinase
Entrez Gene (Human) 945664 (E. coli)
SwissProt (Human) P75959 (E. coli)
Purity Greater than 95% as determined by reducing SDS-PAGE.
Chemical Structure MNHKVHHHHH HMYYGFDIGG TKIALGVFDS GRQLQWEKRV PTPRDSYDAF LDAVCELVAE ADQRFGCKGS VGIGIPGMPE TEDGTLYAAN VPAASGKPLR ADLSARLDRD VRLDNDANCF ALSEAWDDEF TQYPLVMGLI LGTGVGGGLI FNGKPITGKS YITGEFGHMR LPVDALTMMG LDFPLRRCGC GQHGCIENYL SGRGFAWLYQ HYYHQPLQAP EIIALYDQGD EQARAHVERY LDLLAVCLGN ILTIVDPDLV VIGGGLSNFP AITTQLADRL PRHLLPVARV PRIERARHGD AGGMRGAAFL HLTD
Quality Control Endotoxin: Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Directions for Use Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage/Stability Lyophilized protein should be stored at -20 deg C; though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 deg C for 2-7 days. Aliquots of reconstituted samples are stable at -20 deg C for 3 months.
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For research purposes only, not for human use
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