- Cat No.
CRP1442
- Source
Human cells
- Reactivity
Mouse
- Applications
E; WB; SDS-PAGE; MS
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Application key: E- ELISA, WB- Western blot, IH- Immunohistochemistry, IF- Immunofluorescence, FC- Flow cytometry, IC- Immunocytochemistry, IP- Immunoprecipitation, ChIP- Chromatin Immunoprecipitation, EMSA- Electrophoretic Mobility Shift Assay, BL- Blocking, SE- Sandwich ELISA, CBE- Cell-based ELISA, RNAi- RNA interference
Species reactivity key: H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish
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General information
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Description |
Recombinant Mouse DKK1 Protein is produced by our mammalian expression system and the target gene encoding Thr32-His272 is expressed with a 8His tag at the N-terminus. |
Form |
Lyophilized from a 0.2 uM filtered solution of PBS; pH 7.4. |
Gene Symbol |
DKK1 |
Alternative Names |
Dickkopf-related protein 1; Dickkopf-1; Dkk-1; hDkk-1; SK |
Entrez Gene (Mouse) |
13380 |
SwissProt (Mouse) |
O54908 |
Purity |
Greater than 95% as determined by reducing SDS-PAGE. |
Chemical Structure |
HHHHHHHHQT LNSVLINSNA IKNLPPPLGG AGGQPGSAVS VAPGVLYEGG NKYQTLDNYQ PYPCAEDEEC GSDEYCSSPS RGAAGVGGVQ ICLACRKRRK RCMRHAMCCP GNYCKNGICM PSDHSHFPRG EIEESIIENL GNDHNAAAGD GYPRRTTLTS KIYHTKGQEG SVCLRSSDCA AGLCCARHFW SKICKPVLKE GQVCTKHKRK GSHGLEIFQR CYCGEGLACR IQKDHHQASN SSRLHTCQRH |
Quality Control |
Endotoxin: Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. |
Directions for Use |
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Storage/Stability |
Lyophilized protein should be stored at -20 deg C; though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 deg C for 2-7 days. Aliquots of reconstituted samples are stable at -20 deg C for 3 months. |
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