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Recombinant Human CDK6 Protein

  • Cat No.
    CRP1273
  • Source
    E. coli
  • Reactivity
    Human
  • Applications
    E; WB; SDS-PAGE; MS
Application key: E- ELISA, WB- Western blot, IH- Immunohistochemistry, IF- Immunofluorescence, FC- Flow cytometry, IC- Immunocytochemistry, IP- Immunoprecipitation, ChIP- Chromatin Immunoprecipitation, EMSA- Electrophoretic Mobility Shift Assay, BL- Blocking, SE- Sandwich ELISA, CBE- Cell-based ELISA, RNAi- RNA interference

Species reactivity key: H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish
General information
Specifications
Data
References
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Description Recombinant Human CDK6 Protein is produced by our E. coli expression system and the target gene encoding Met1-Ala326 is expressed with a 6His tag at the N-terminus.
Form Liquid in a 0.2 uM filtered solution of 50mM Tris; 100mM NaCl; 20% Glycerol; 5mM DTT; pH 8.0.
Gene Symbol CDK6
Alternative Names CDKN6; Cyclin-dependent kinase 6; Cell division protein kinase 6; Serine/threonine-protein kinase PLSTIRE
Entrez Gene (Human) 1021
SwissProt (Human) Q00534
Purity Greater than 95% as determined by reducing SDS-PAGE.
Chemical Structure MGSSHHHHHH SSGLVPRGSH MEKDGLCRAD QQYECVAEIG EGAYGKVFKA RDLKNGGRFV ALKRVRVQTG EEGMPLSTIR EVAVLRHLET FEHPNVVRLF DVCTVSRTDR ETKLTLVFEH VDQDLTTYLD KVPEPGVPTE TIKDMMFQLL RGLDFLHSHR VVHRDLKPQN ILVTSSGQIK LADFGLARIY SFQMALTSVV VTLWYRAPEV LLQSSYATPV DLWSVGCIFA EMFRRKPLFR GSSDVDQLGK ILDVIGLPGE EDWPRDVALP RQAFHSKSAQ PIEKFVTDID ELGKDLLLKC LTFNPAKRIS AYSALSHPYF QDLERCKENL DSHLPPSQNT SELNTA
Quality Control Endotoxin: Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Directions for Use Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage/Stability Store it at -20 deg C to -80 deg C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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For research purposes only, not for human use
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