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Recombinant Human BDNF Protein

  • Cat No.
    CRP1113
  • Source
    E. coli
  • Reactivity
    Human
  • Applications
    E; WB; SDS-PAGE; MS
Application key: E- ELISA, WB- Western blot, IH- Immunohistochemistry, IF- Immunofluorescence, FC- Flow cytometry, IC- Immunocytochemistry, IP- Immunoprecipitation, ChIP- Chromatin Immunoprecipitation, EMSA- Electrophoretic Mobility Shift Assay, BL- Blocking, SE- Sandwich ELISA, CBE- Cell-based ELISA, RNAi- RNA interference

Species reactivity key: H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish
General information
Specifications
Data
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Description Recombinant Human BDNF Protein is produced by E. coli. It is a single non-glycosylated polypeptide chain containing 119 amino acids. Bioactive BDNF is a non-covalently linked homodimer with a molecular mass of 27 kD.
Form Lyophilized from a 0.2 uM filtered solution of 20mM PB; 250mM NaCl; pH 7.2.
Gene Symbol BDNF
Alternative Names Brain-derived neurotrophic factor; BDNF; Abrineurin
Entrez Gene (Human) 627
SwissProt (Human) P23560
Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Chemical Structure MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR
Quality Control Endotoxin: Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Directions for Use Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage/Stability Lyophilized protein should be stored at -20 deg C; though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 deg C for 2-7 days. Aliquots of reconstituted samples are stable at -20 deg C for 3 months.
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For research purposes only, not for human use
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