ssc-mir-376c RT-PCR Primer Set

Description	Stem-loop RT primer and PCR primer set to detect mature miRNA ssc-mir-376c expression.
Form	Lyophilized powder
Directions for Use	RT primer (1 nmol): Add 100 ul RNase-free H2O to 10 uM RT primer dilution. PCR primer set (2 nmol): Add 200 ul RNase-free H2O to 10 uM primer set dilution.
PCR Method	2-Step qRT-PCR
Detection Range	Seven orders of magnitude
Sensitivity	10 copies of a standard mimics and as little as 25 pg of total RNA
Sample Types	miRNA; Total RNA
Platform	ABI 7500 System/ViiA 7 System/StepOne/StepOnePlus/7900HT System; BioRad IQ5.0/CFX Connect; Stratagen MX3000p/4000p; Roche LightCycler 480
Application	The miRNAs RT-PCR primer set contains a stem-loop RT primer and a pair of specific PCR primer set. Combined with the custom's RT and PCR reagent. It provides a sensitive and specific method of real-time PCR for quantificating of miRNAs from total RNA samples. It is Ideal for validating microRNA expression profiling data and high-throughput applications. Trait lies in even highly homogized miRNAs can be accurately dicriminated ensurenced by stemloop RT primer and high specific miRNAs primer set.
Storage/Stability	-20 C for 2 years