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ssc-mir-376c RT-PCR Primer Set

  • Cat No.
    CPK3377
  • Source
    Synthetic
  • Reactivity
    P
  • Applications
    RT-PCR; Real-time RT-PCR
Application key: E- ELISA, WB- Western blot, IH- Immunohistochemistry, IF- Immunofluorescence, FC- Flow cytometry, IC- Immunocytochemistry, IP- Immunoprecipitation, ChIP- Chromatin Immunoprecipitation, EMSA- Electrophoretic Mobility Shift Assay, BL- Blocking, SE- Sandwich ELISA, CBE- Cell-based ELISA, RNAi- RNA interference

Species reactivity key: H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish
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Description Stem-loop RT primer and PCR primer set to detect mature miRNA ssc-mir-376c expression.
Form Lyophilized powder
Directions for Use RT primer (1 nmol): Add 100 ul RNase-free H2O to 10 uM RT primer dilution. PCR primer set (2 nmol): Add 200 ul RNase-free H2O to 10 uM primer set dilution.
PCR Method 2-Step qRT-PCR
Detection Range Seven orders of magnitude
Sensitivity 10 copies of a standard mimics and as little as 25 pg of total RNA
Sample Types miRNA; Total RNA
Platform ABI 7500 System/ViiA 7 System/StepOne/StepOnePlus/7900HT System; BioRad IQ5.0/CFX Connect; Stratagen MX3000p/4000p; Roche LightCycler 480
Application The miRNAs RT-PCR primer set contains a stem-loop RT primer and a pair of specific PCR primer set. Combined with the custom's RT and PCR reagent. It provides a sensitive and specific method of real-time PCR for quantificating of miRNAs from total RNA samples. It is Ideal for validating microRNA expression profiling data and high-throughput applications. Trait lies in even highly homogized miRNAs can be accurately dicriminated ensurenced by stemloop RT primer and high specific miRNAs primer set.
Storage/Stability -20 C for 2 years
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For research purposes only, not for human use
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