Description: Recombinant Human CD274 Protein is produced by mammalian expression system and the target gene encoding Phe19-Thr239 is expressed with a 6His tag at the C-terminus. Form: Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, 5% Trehalose, pH 7.4. Gene Symbol: CD274 Alternative Names: B7H1; PDCD1L1; PDCD1LG1; PDL1; Programmed cell death 1 ligand 1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7 homolog 1; B7-H1; CD274Entrez Gene (Human): 29126SwissProt (Human): Q9NZQ7Purity : Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure : FTVTVPKDLY VVEYGSNMTI ECKFPVEKQL DLAALIVYWE MEDKNIIQFV HGEEDLKVQH SSYRQRARLL KDQLSLGNAA LQITDVKLQD AGVYRCMISY GGADYKRITV KVNAPYNKIN QRILVVDPVT SEHELTCQAE GYPKAEVIWT SSDHQVLSGK TTTTNSKREE KLFNVTSTLR INTTTNEIFY CTFRRLDPEE NHTAELVIPE LPLAHPPNER TVDHHHHHHDirections for Use : Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control : Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability : Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

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  Immobilized Anti-CD274 antibody at 2 μg/ml (100 μl/well) can bind Recombinant Human CD274 Protein. The ED50 of Recombinant Human CD274 Protein is 0.0094 μg/ml.