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Recombinant Human ATG5 Protein CRP1091
Product NameRecombinant Human ATG5 Protein
Cat No: CRP1091
Source: E. coli
Reactivity: Human
Applications: E, WB, SDS-PAGE, MS
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
10 μg
$140
50 μg
$420
500 μg
$2100
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Description: Recombinant Human ATG5 Protein is produced by E. coli expression system and the target gene encoding Met1-Asp275 is expressed.
Form: Liquid in a 0.2 μM filtered solution of 20mM Tris, 0.2M NaCl, 1mM DTT, 40% Glycerol, pH 8.0.
Gene Symbol: ATG5
Alternative Names: APG5L; ASP; Autophagy protein 5; APG5-like; Apoptosis-specific protein
Entrez Gene (Human): 9474
SwissProt (Human): Q9H1Y0
Purity : Greater than 95% as determined by reducing SDS-PAGE.
Chemical Structure : MTDDKDVLRD VWFGRIPTCF TLYQDEITER EAEPYYLLLP RVSYLTLVTD KVKKHFQKVM RQEDISEIWF EYEGTPLKWH YPIGLLFDLL ASSSALPWNI TVHFKSFPEK DLLHCPSKDA IEAHFMSCMK EADALKHKSQ VINEMQKKDH KQLWMGLQND RFDQFWAINR KLMEYPAEEN GFRYIPFRIY QTTTERPFIQ KLFRPVAADG QLHTLGDLLK EVCPSAIDPE DGEKKNQVMI HGIEPMLETP LQWLSEHLSY PDNFLHISII PQPTD
Directions for Use : Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Quality Control : Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Storage/Stability : Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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