Description: Recombinant Human ARHGAP25 Protein is produced by mammalian expression system and the target gene encoding Met1-Leu458 is expressed with a 6His tag at the C-terminus. Form: Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 1 mM DTT, 5 mM MgCl2, pH 7.5. Gene Symbol: ARHGAP25 Alternative Names: KIAA0053; Rho GTPase-activating protein 25; Rho-type GTPase-activating protein 25Entrez Gene (Human): 9938SwissProt (Human): P42331Purity : Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure : MSLGQSACLF LSIARSRSVM TGEQMAAFHP SSTPNPLERP IKMGWLKKQR SIVKNWQQRY FVLRAQQLYY YKDEEDTKPQ GCMYLPGCTI KEIATNPEEA GKFVFEIIPA SWDQNRMGQD SYVLMASSQA EMEEWVKFLR RVAGTPCGAV FGQRLDETVA YEQKFGPHLV PILVEKCAEF ILEHGRNEEG IFRLPGQDNL VKQLRDAFDA GERPSFDRDT DVHTVASLLK LYLRDLPEPV VPWSQYEGFL LCGQLTNADE AKAQQELMKQ LSILPRDNYS LLSYICRFLH EIQLNCAVNK MSVDNLATVI GVNLIRSKVE DPAVIMRGTP QIQRVMTMMI RDHEVLFPKS KDIPLSPPAQ KNDPKKAPVA RSSVGWDATE DLRISRTDSF SSMVRCREPS CFHWVLPLVQ AIPCKACSRV AIWGVLGDAV AVGAAATDSS EHTLKAWPLS KSSFYWHLVD HHHHHH Directions for Use : Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control : Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability : Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.