Description: Rabbit polyclonal antibody to MFN1 Immunogen: Recombinant full length protein of human MFN1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: MFN1 Alternative Names: Mitofusin-1; Fzo homolog; Transmembrane GTPase MFN1Entrez Gene (Human): 55669Entrez Gene (Mouse) : 67414Entrez Gene (Rat) : 192647SwissProt (Human): Q8IWA4SwissProt (Mouse) : Q811U4SwissProt (Rat) : Q8R4Z9Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of MFN1 expression in A549 (A), mouse liver (B), rat brain (C) whole cell lysates.
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  Immunofluorescent analysis of MFN1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).