Description: Rabbit polyclonal antibody to MBP Immunogen: Recombinant full length protein of human MBP Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: MBP Alternative Names: Myelin basic protein; MBP; Myelin A1 protein; Myelin membrane encephalitogenic proteinEntrez Gene (Human): 4155Entrez Gene (Mouse) : 17196Entrez Gene (Rat) : 24547SwissProt (Human): P02686SwissProt (Mouse) : P04370SwissProt (Rat) : P02688Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of MBP expression in SHSY5Y (A), HT29 (B), PC12 (C), mouse brain (D) whole cell lysates.
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Immunohistochemical analysis of MBP staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.