Description: Rabbit polyclonal antibody to DHODH Immunogen: Recombinant full length protein of human DHODH Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: DHODH Alternative Names: Dihydroorotate dehydrogenase (quinone) mitochondrial; DHOdehase; Dihydroorotate oxidaseEntrez Gene (Human): 1723Entrez Gene (Mouse) : 56749Entrez Gene (Rat) : 65156SwissProt (Human): Q02127SwissProt (Mouse) : O35435SwissProt (Rat) : Q63707Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of DHODH expression in MCF7 (A), HL60 (B), SW620 (C), mouse spleen (D), rat kidney (E) whole cell lysates.
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  Immunohistochemical analysis of DHODH staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.