Description: Rabbit polyclonal antibody to Histone H3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Histone H3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: HIST1H3A Alternative Names: H3FA; H3FL; H3FC; H3FB; H3FD; H3FI; H3FH; H3FK; H3FF; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/lEntrez Gene (Human): 8350; 8351; 8352; 8353; 8354; 8355; 8356; 8357; 8358; 8968Entrez Gene (Mouse) : 319152; 319153; 360198; 97908Entrez Gene (Rat) : 291159; 682330SwissProt (Human): P68431SwissProt (Mouse) : P68433SwissProt (Rat) : Q6LED0Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Histone H3 expression in zebrafish (A) whole cell lysates.
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  Immunohistochemical analysis of Histone H3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.