Description: Rabbit polyclonal antibody to FTO Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human FTO. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: FTO Alternative Names: KIAA1752; Alpha-ketoglutarate-dependent dioxygenase FTO; Fat mass and obesity-associated proteinEntrez Gene (Human): 79068Entrez Gene (Mouse) : 26383Entrez Gene (Rat) : 291905SwissProt (Human): Q9C0B1SwissProt (Mouse) : Q8BGW1SwissProt (Rat) : Q2A121Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of FTO expression in HEK293T (A), Hela (B), A549 (C), rat lung (D), rat kidney (E) whole cell lysates.
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  Immunofluorescent analysis of FTO staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).