Description: Rabbit polyclonal antibody to PDK1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PDK1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000) Gene Symbol: PDK1 Alternative Names: PDHK1; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1 mitochondrial; Pyruvate dehydrogenase kinase isoform 1; PDH kinase 1Entrez Gene (Human): 5163SwissProt (Human): Q15118Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PDK1 expression in H1792 (A), CT26 (B) whole cell lysates.
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  Immunohistochemical analysis of PDK1 staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.