Recombinant Anti-CALU Rabbit mAb-Primary Antibody-Cohesion Biosciences

Western blot analysis of CALU expression in K562 (A) whole cell lysates. (Predicted band size: 37 kD; Observed band size: 47 kD)
Immunohistochemical analysis of CALU staining in human squamous cell carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).
Immunofluorescent analysis of CALU staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Name	Recombinant Anti-CALU Rabbit mAb
Cat No:	CMA4350
Source:	Rabbit
Reactivity:	H
Applications:	WB, IH, IF/IC
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish

Size

Price

200 μl

$350

100 μl

$220

30 μl

$110

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Description: Recombinant rabbit monoclonal antibody to CALU

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human CALU. The exact sequence is proprietary.

Purification: The antibody was purified by immunogen affinity chromatography.

Clonality: Monoclonal

Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene Symbol: CALU

Alternative Names: Calumenin; Crocalbin; IEF SSP 9302

Entrez Gene (Human): 813

SwissProt (Human): O43852

Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Western blot analysis of CALU expression in K562 (A) whole cell lysates. (Predicted band size: 37 kD; Observed band size: 47 kD)
Immunohistochemical analysis of CALU staining in human squamous cell carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).
Immunofluorescent analysis of CALU staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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