Description: Recombinant rabbit monoclonal antibody to Flotillin-1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Flotillin-1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: FLOT1 Alternative Names: Flotillin-1Entrez Gene (Human): 10211Entrez Gene (Mouse) : 14251Entrez Gene (Rat) : 64665SwissProt (Human): O75955SwissProt (Mouse) : O08917SwissProt (Rat) : Q9Z1E1Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Flotillin-1 expression in Hela (A), A549 (B), mouse liver (C), mouse kidney (D), rat liver (E), rat kidney (F) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 48 kD)
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  Immunohistochemical analysis of Flotillin-1 staining in human ovary formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).