Description: Recombinant rabbit monoclonal antibody to SAP61 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human SAP61. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: SF3A3 Alternative Names: SAP61; Splicing factor 3A subunit 3; SF3a60; Spliceosome-associated protein 61; SAP 61Entrez Gene (Human): 10946Entrez Gene (Mouse) : 75062SwissProt (Human): Q12874SwissProt (Mouse) : Q9D554Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of SAP61 expression in HEK293T (A), Hela (B), K562 (C), mouse muscle (D) whole cell lysates. (Predicted band size: 58 kD; Observed band size: 59 kD)
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  Immunohistochemical analysis of SAP61 staining in human squamous cell carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).