Description: Recombinant rabbit monoclonal antibody to p115 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human p115. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: USO1 Alternative Names: VDP; General vesicular transport factor p115; Protein USO1 homolog; Transcytosis-associated protein; TAP; Vesicle-docking proteinEntrez Gene (Human): 8615SwissProt (Human): O60763Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of p115 expression in HEK293T (A), A549 (B), HepG2 (C) whole cell lysates. (Predicted band size: 107 kD; Observed band size: 108 kD)
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  Immunohistochemical analysis of p115 staining in human squamous cell carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).