Description: Recombinant rabbit monoclonal antibody to Estrogen Receptor alpha Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Estrogen Receptor alpha. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: ESR1 Alternative Names: ESR; NR3A1; Estrogen receptor; ER; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1Entrez Gene (Human): 2099Entrez Gene (Mouse) : 13982Entrez Gene (Rat) : 24890SwissProt (Human): P03372SwissProt (Mouse) : P19785SwissProt (Rat) : P06211Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Estrogen Receptor alpha expression in H1792 (A), Myla2059 (B), LO2 (C), mouse liver (D), rat liver (E) whole cell lysates. (Predicted band size: 66 kD; Observed band size: 60 kD)
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  Immunohistochemical analysis of Estrogen Receptor alpha staining in human squamous cell carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).