Description: Recombinant rabbit monoclonal antibody to IBA1 Immunogen: KLH-conjugated synthetic peptide of human IBA1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS, pH 7.3, 50% glycerol, and 0.05% Proclin300. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: AIF1 Alternative Names: G1; IBA1; Allograft inflammatory factor 1; AIF-1; Ionized calcium-binding adapter molecule 1; Protein G1Entrez Gene (Human): 199Entrez Gene (Mouse) : 11629Entrez Gene (Rat) : 29427SwissProt (Human): P55008SwissProt (Mouse) : O70200SwissProt (Rat) : P55009Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of IBA1 expression in Myla2059 (A), THP1 (B), mouse brain (C), mouse kidney (D), rat brain (E) whole cell lysates. (Predicted band size: 16 kD; Observed band size: 16 kD)
• 
  Immunofluorescent analysis of IBA1 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.