Description: Rabbit monoclonal antibody to TCF7L2 Immunogen: A synthetic peptide of human TCF-4 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/10 - 1/50) Gene Symbol: TCF7L2 Alternative Names: TCF4; Transcription factor 7-like 2; HMG box transcription factor 4; T-cell-specific transcription factor 4; T-cell factor 4; TCF-4; hTCF-4Entrez Gene (Human): 6934SwissProt (Human): Q9NQB0Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of TCF7L2 expression in K562 (A), Hela (B) whole cell lysates.
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Immunohistochemical analysis of TCF7L2 staining in human cholangiocarcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.