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ECL Detection Reagent CRG1026
Product NameECL Detection Reagent
Cat No: CRG1026
Reactivity: N/A
Applications: WB
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
30 ml
60 ml
120 ml
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Description: ECL Detection Reagent is suitable for all routine Western blotting
Form: Liquid
Directions for Use : Mix ECL Detection Reagent A and B at 1:1. Use 0.1 ml mix solution per cm2 of membrane. For best results prepare mix solution immediately before use. The mix solution is stable for up to 1 hour at room temperature in dark.
Components : ECL Detection Reagent A, ECL Detection Reagent B
Application : ECL Detection Reagent uses horseradish peroxidase (HRP) conjugated anti-mouse and anti-rabbit antibodies for luminol-based detection of Western blots. Blots probed with mouse or rabbit primary antibodies are incubated with HRP-conjugated secondary antibodies. Addition of ECL Detection Reagent results in a chemiluminescent signal that can be captured on film or detected with a CCD camera.
Storage/Stability : Store at 4°C for one year. Do not freeze.
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