Description: ECL Detection Reagent is suitable for all routine Western blotting Form: Liquid Directions for Use : Mix ECL Detection Reagent A and B at 1:1. Use 0.1 ml mix solution per cm2 of membrane. For best results prepare mix solution immediately before use. The mix solution is stable for up to 1 hour at room temperature in dark.Components : ECL Detection Reagent A, ECL Detection Reagent BApplication : ECL Detection Reagent uses horseradish peroxidase (HRP) conjugated anti-mouse and anti-rabbit antibodies for luminol-based detection of Western blots. Blots probed with mouse or rabbit primary antibodies are incubated with HRP-conjugated secondary antibodies. Addition of ECL Detection Reagent results in a chemiluminescent signal that can be captured on film or detected with a CCD camera.Storage/Stability : Store at 4°C for one year. Do not freeze.