Description:Rabbit polyclonal antibody to CingulinImmunogen:Recombinant fusion protein of human Cingulin. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IH (1/50 - 1/200)Gene Symbol:CGNAlternative Names:KIAA1319; Cingulin
Entrez Gene (Human):
57530;
SwissProt (Human):
Q9P2M7;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Cingulin expression in LO2 (A), A549 (B) whole cell lysates. (Predicted band size: 87; 136 kD; Observed band size: 160 kD)
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Immunohistochemical analysis of Cingulin staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.