Description：Rabbit polyclonal antibody to PUMAImmunogen：KLH-conjugated synthetic peptide of human PUMA. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol：BBC3Alternative Names：PUMA; Bcl-2-binding component 3; JFY-1; p53 up-regulated modulator of apoptosis Entrez Gene (Human)： 27113; Entrez Gene (Mouse)： 170770; Entrez Gene (Rat)： 317673; SwissProt (Human)： Q9BXH1; Q96PG8; SwissProt (Mouse)： Q99ML1; SwissProt (Rat)： Q80ZG6; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PUMA expression in HepG2 (A), K562 (B) whole cell lysates. (Predicted band size: 10; 14; 20; 26 kD; Observed band size: 20 kD)
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  Immunofluorescent analysis of PUMA staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).