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IRS1 (pS312) Blocking Peptide CBP5091
  • Western blot analysis of IRS1 (pS312) expression in HepG2 (A), Hela (B), mouse spleen (C) whole cell lysates.
  • Immunofluorescent analysis of IRS1 (pS312) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Product NameIRS1 (pS312) Blocking Peptide
Cat No: CBP5091
Source: Synthetic
Reactivity: H, M, R
Applications: BL
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
1 mg
$150
5 mg
$450
Add to cart Ship in 3 weeks My orders
Datasheet
MSDS
Description: The peptide is used to block Anti-IRS1 (pS312) Antibody (#CPA5091) reactivity.
Form: Lyophilized powder
Gene Symbol: IRS1
Alternative Names: Insulin receptor substrate 1; IRS-1
Entrez Gene (Human): 3667
Entrez Gene (Mouse) : 16367
Entrez Gene (Rat) : 25467
SwissProt (Human): P35568
SwissProt (Mouse) : P35569
SwissProt (Rat) : P35570
Purity : >85%
Directions for Use : Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4°C for overnight or at room temperature for 2 hours.
Quality Control : The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
Storage/Stability : Shipped at 4°C. Store at -20°C for one year.
  • Western blot analysis of IRS1 (pS312) expression in HepG2 (A), Hela (B), mouse spleen (C) whole cell lysates.
  • Immunofluorescent analysis of IRS1 (pS312) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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